Ef1a short promoter. Promoters of mammalian origin have been used most widely for this purpose, among which the human eukaryotic translation elongation factor 1 alpha (EF‐1α, gene symbol EEF1A1) promoter is constitutively active in a broad range of cell types. The Pribnow box (TATAAT) is located at the -10 position and is essential for transcription initiation. Provides stable, constitutive expression with reduced silencing compared to viral promoters like CMV. Optimal promoter selection can enhance target specificity and gene expression. The exact activity of the transgene depends on the promoter chosen, so the choice of promoter is one of the critical decisions when designing a genetic Elongation factor 1-alpha 1 (eEF1a1) is a translation elongation protein, expressed across eukaryotes. The three promoters were put into the same expression vector, pAC-luc, driving expression of the luciferase cDNA. [5][6] This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. Researchers have engineered smaller versions, like the EF1a short promoter (EFS), which are more easily packaged into viral vectors. Nov 2, 2016 · Furthermore, the interaction of the GmScreamM8 leader intron with regulatory element sequences from several soybean eEF1A promoters was studied using synthetic promoters, which consisted of element tetramers upstream of a core promoter used to regulate a green fluorescent protein (gfp) reporter gene. Connie Cepko's lab contains the insert EF1 alpha promoter and is published in Proc Natl Acad Sci U S A. Compact promoter derived from human elongation factor 1-alpha. It is a cellular-derived enhancer/promoter with decreased cross-activation of nearby promoters, therefore hypothetically decreasing the risk of genotoxicity. Mar 7, 2014 · Abstract The promoter is a major element in the expression cassette of gene therapy vectors. Keywords: EF1a promoter, Transgenesis, Pronuclear microinjection, DNA methylation, Expression Introduction Transgenic animals are a powerful tool for the study of many biological processes. Recently, we evaluated three different human elongation factor 1 alpha (EF1α) promoters. The exact activity of the transgene depends on the promoter chosen, so the choice of promoter is one of the critical decisions when designing a genetic Oct 22, 2025 · Bacterial Promoters Promoters in bacteria contain two short DNA sequences located at the -10 (10 bp 5' or upstream) and -35 positions from the transcription start site (TSS) (Figure 2). 2004 Jan 6. TEF1 promoter Translation elongation factor 1 alpha (Tef1) is a crucial component of the eukaryotic translation machinery and mediates the delivery of aminoacyl tRNAs to the ribosomes to sustain the elongation of the peptide chain [95]. 客服邮箱： service@x-mol. 101 (1):16-22. Sep 5, 2017 · Of the six promoters, the CHEF-1α promoter yielded the highest transgene expression levels, whereas the CMV promoter maintained transgene expression more stably during long-term culture of cells. Apr 20, 2017 · The elongation factor 1α short (EFS) promoter mediated the most robust expression, whereas expression levels were lower from the potent but more silencing-prone spleen focus forming virus (SFFV Jun 23, 2017 · Why is there a difference between the EF1a promoter on different vectors? As an example, the pEF vector available from Sigma, as well as most EF1 vectors on addgene have a 1172 bp EF1a promoter Jun 21, 2025 · The promoter is also used to create stable cell lines that continuously produce a protein of interest for study. This plasmid is available through Addgene. Dec 6, 2014 · EF1alpha (or its short, intron-less form, EFS) is a promoter that has been recently used in many clinical trials. com 官方微信：X-molTeam2 邮编：100098 地址：北京市海淀区知春路56号中航科技大厦. Plasmid pEF-GFP from Dr. We would like to show you a description here but the site won’t allow us. Dec 6, 2014 · EF1alpha (or its short, intron-less form, EFS) is a promoter that has been recently used in many clinical trials. Lentiviral vector systems with EF-1 alpha promoters allow you to achieve robust, constitutive, long-term expression of your gene of interest in cell types in which CMV promoters are often silenced, such as hematopoietic and stem cells. In humans, it is encoded by the EEF1A1 gene. The activity from Dec 6, 2014 · Request PDF | Systematic Comparison of the EF-1 Alpha Short (EFS) and Viral Promoters for Gene Modification of Human Primary Cells for Clinical Applications | Optimization of transgene expression Feb 14, 2002 · In the present study, we compared the murine cytomegalovirus immediate early (MCMV IE) promoter and the human cytomegalovirus immediate early (HCMV IE) promoter containing the short stretch of 5′ untranslated region (UTR) in the absence or presence of the human elongation factor one alpha (EF-1α) first intron to direct foreign gene Keywords: EF1a promoter, Transgenesis, Pronuclear microinjection, DNA methylation, Expression Introduction Transgenic animals are a powerful tool for the study of many biological processes.